Elucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics

Cho 8_8

As no standard chemotherapy regimen has been established for advanced gastric cancer, this study sought to evaluate the efficacy and safety of combination chemotherapy that included paclitaxel and leucovorin (LV)-modulated infusional 5-fluorouracil (5-FU) in metastatic gastric cancer. Patients received a three-hour infusion of 175 mg/m2 of paclitaxel on day 1. A bolus of 20 mg/m2 of LV was then...

CHO cell lines generated by PiggyBac transposition

A major bottleneck in the manufacture of recombinant therapeutic proteins is the time and effort needed for the generation of stable, high-producing mammalian cell lines. Conventional gene transfer methods for stable cell line generation rely on random transgene integration, resulting in unpredictable and highly variable levels of expression of the transgene in individual clones [1,2]. As a con...

Won - Seob Cho 2005

Effects of carbohydrate (CHO) and fat supplementation on CHO metabolism during prolonged exercise.

The aim of the study was to examine carbohydrate (CHO) utilization in subjects receiving CHO or CHO + medium-chain triglycerides (MCT) supplements during 180 minutes of exercise at 50% maximal aerobic work rate ([Wmax] 57% maximal oxygen consumption [VO2max]). In a double-blind crossover design, nine trained athletes cycled four times. Subjects received a bolus of 4 mL x kg(-1) at the start and...

DNA methylation in CHO cells.

Chinese hamster ovary (CHO) cells account for the production of the majority of biopharmaceutical molecules - however, the molecular basis for their versatile properties is not entirely understood yet and the underlying cellular processes need to be characterized in detail. One such process that is supposed to contribute significantly to CHO cell phenotype is methylation of DNA at cytosine resi...